High‑affinity cyclic peptides can be identified for multi‑pass membrane proteins with high reliability. A cell‑based screening approach enables evaluation in functionally relevant target states. This approach helps address key limitations of conventional discovery methods.
Peptides with wide varieties and high-affinities (~nM) can be obtained with high success rate.
- Proprietary mRNA display methods enable efficient screening.
- Practical biosynthesis & assays enable rapid selection and activity explorations.
- Innovative improvements in mRNA display enable screening from >1013 peptides.
- Practical biosynthesis & assays enable rapid selection and activity explorations.
Peptides with wide varieties and high-affinities(~nM) can be obtained with high success rate (If you are interested in details of our track record, please feel free to contact us.).
Hit peptides have been obtained toward >20 targets with our platform.
High binding affinity was achieved as the Hits in initial screening.
Peptides are gathering significant attention not only for their affinity and specificity towards a diverse array of target proteins but also for their tissue permeability (oral suitability) and ease of chemical synthesis. Notably, cyclic peptides that incorporate unnatural amino acids are expected as PPI inhibitors. Additionally, they are anticipated to act as DDS ligands, facilitating the delivery of nucleic acid-based medicines and other drugs to targeted tissues/cells due to their exceptional binding affinity and stability.
Fujifilm offers a comprehensive suite of contract services. Our core offerings include the use of mRNA display technology to discover peptides that bind strongly and specifically to target proteins, combined with rapid structural optimization and activity screening of these hit peptides (e.g., inhibitory activity, selectivity). Additionally, we provide supporting services such as peptide chemical synthesis and the preparation of target proteins.
- Improved mRNA display allows screening from a huge library.
- Many conditions (=cycle size, targets, selections) can be performed in parallel.
A variety of hits have been obtained with a high success rate.
Rapid selection and activity exploration by practical biosynthesis & assay combined with conventional chemical synthesis.
Chemical Synthesis: long lead time (20~30days) and not suited to parallel synthesis/purification.
Biosynthesis: short lead time (5~7days) and suited for parallel preparation (~1,000 peptides/month)
Diverse binding peptides were successfully obtained toward both of 2 FGFRs (fibroblast growth factor receptor).
- FGFR activatable peptide had been found through rapid and exhaustive cell activity screening with biosynthetic peptides from diverse hit peptides.
- Optimized peptide showed same cell growth rate with the natural protein (bFGF) in MSC (Mesenchymal Stem Cell).
- siRNAs were conjugated to the integrin αvβ3-binding peptide. With this Peptide Oligonucleotide Conjugates, cell-selective target gene knockdown were achieved.
- Peptide Discovery Platform for Advancing Peptide-Oligonucleotide Conjugation (Yukiko Ishii, Shin Izuta, Masahiro Kochi, Koo Suzuki)
- Peptide Discovery Platform for Advancing Peptide-Oligonucleotide Conjugation (Yukiko Ishii, Shin Izuta, Masahiro Kochi, Koo Suzuki)
- Cyclic Peptide Discovery Services (Yukiko Ishii, Shin Izuta, Ryota Haba)